Session 5
8 Feb 2021
4:30 - 6:00pm SGT
5:30 - 7:00pm JST
7:30-9:00pm AEST
8 Feb 2021
4:30 - 6:00pm SGT
5:30 - 7:00pm JST
7:30-9:00pm AEST
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Allison COWIN
Development of cell-based therapies for wound repair |
Abstract
Stem cell-based therapies have been shown to have considerable potential for improving wound healing however delivering viable cells to wounds is fraught with difficulties particularly in a clinical setting. We are developing two approaches to improve the use of stem cells for the treatment of wounds. The first is the development of CyPatch; a wound dressing that can support and deliver healthy stem cells into wounds, the second approach is to investigate a cell-free therapy that uses stem cell secretome for the treatment of wounds. Our CyPatch technology uses plasma polymerization to functionalize a surface that enables cellular attachment and delivery into wounds. Using murine and porcine models of wound healing we have tested this dressing which has now entered Phase I/II clinical trials in diabetic foot ulcers. The effectiveness of using stem cell secretome as a potential cell-free therapy has also been investigated. Using both in vitro and in vivo wound models we have shown that the secretome improves healing with decreased inflammation and improved collagen deposition. This presentation will overview the use of stem cells for the treatment of wounds and will present the data from our CyPatch preclinical trials as well as our secretome studies.
Stem cell-based therapies have been shown to have considerable potential for improving wound healing however delivering viable cells to wounds is fraught with difficulties particularly in a clinical setting. We are developing two approaches to improve the use of stem cells for the treatment of wounds. The first is the development of CyPatch; a wound dressing that can support and deliver healthy stem cells into wounds, the second approach is to investigate a cell-free therapy that uses stem cell secretome for the treatment of wounds. Our CyPatch technology uses plasma polymerization to functionalize a surface that enables cellular attachment and delivery into wounds. Using murine and porcine models of wound healing we have tested this dressing which has now entered Phase I/II clinical trials in diabetic foot ulcers. The effectiveness of using stem cell secretome as a potential cell-free therapy has also been investigated. Using both in vitro and in vivo wound models we have shown that the secretome improves healing with decreased inflammation and improved collagen deposition. This presentation will overview the use of stem cells for the treatment of wounds and will present the data from our CyPatch preclinical trials as well as our secretome studies.
Bio
Allison Cowin is a leader in wound healing, internationally renowned for her work on the cytoskeletal protein Flightless I and how it negatively impacts on tissue repair. She is an NHMRC Senior Research Fellow and Professor of Regenerative Medicine at the Future Industries Institute UniSA, leading a group of 12 research scientists and students investigating all aspects of wound healing and regenerative biology. She has been awarded over $16M in grants including from the US Department of Defence and continuous NHMRC funding since 2004 for her work developing antibody technologies for the treatment of wounds. Her research has received special recognition from the NHMRC by inclusion in its ‘10 of the best projects’ publication. She has been awarded five independent fellowships (3 from the NHMRC) won the Women in Innovation: Science award (2016) was an SA finalist in the Telstra Women’s Business awards in 2015 and her research “Novel drug delivery of therapeutic antibodies to wounds” made the finals of the “Australian Innovation Challenge” (2015). Prof Cowin is the Co-Chair of the National AHTA Wound Research Initiative and is the Editor of the Australian journal Wound Practice & Research. Prof Cowin was the founder and inaugural President (2007-2012) of the Australasian Wound & Tissue Repair Society and is now Treasurer of this organisation. She is currently President Elect and Secretary of the Australasian Society for Dermatology Research.
Allison Cowin is a leader in wound healing, internationally renowned for her work on the cytoskeletal protein Flightless I and how it negatively impacts on tissue repair. She is an NHMRC Senior Research Fellow and Professor of Regenerative Medicine at the Future Industries Institute UniSA, leading a group of 12 research scientists and students investigating all aspects of wound healing and regenerative biology. She has been awarded over $16M in grants including from the US Department of Defence and continuous NHMRC funding since 2004 for her work developing antibody technologies for the treatment of wounds. Her research has received special recognition from the NHMRC by inclusion in its ‘10 of the best projects’ publication. She has been awarded five independent fellowships (3 from the NHMRC) won the Women in Innovation: Science award (2016) was an SA finalist in the Telstra Women’s Business awards in 2015 and her research “Novel drug delivery of therapeutic antibodies to wounds” made the finals of the “Australian Innovation Challenge” (2015). Prof Cowin is the Co-Chair of the National AHTA Wound Research Initiative and is the Editor of the Australian journal Wound Practice & Research. Prof Cowin was the founder and inaugural President (2007-2012) of the Australasian Wound & Tissue Repair Society and is now Treasurer of this organisation. She is currently President Elect and Secretary of the Australasian Society for Dermatology Research.
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Akira SATOH
Dermal collagen structure of the axolotl skin |
Abstract
Axolotls, Ambystoma mexicanum, have a remarkable organ-level regeneration ability. Their regeneration ability has fascinated scientists for a long time since mammals, including humans, do not have it. The biggest issue in this field was “what are the inductive molecules?” This proposition was conceptually made in 1823. We recently succeeded in identifying the inductive molecules by using a unique experimental model. The identified molecules are BMP2+FGF2+FGF8. These regeneration inducers actually can induce organ-level regeneration in not only limbs but also the tail, teeth, and gills. Moreover, the molecules can induce limb regeneration in newts, Pleurodeles waltl, and froglets, Xenopus laevis. Indeed, our defined molecules are the regeneration inductive molecules for multiple species and multiple organs. Due to the achievement, a comparative approach can be taken at the molecular level among axolotl organs. Base on the achievement as above, we have just started investigating axolotl skin regeneration. Skin regeneration has been a gigantic research field. But unexpectedly, collagen synthetic cells in the dermis appear not to be well clarified in any species as far as we googled and PubMed-surfed. We started our research from describing collagen synthetic cells in the axolotl skin. And……My vocabulary is not sufficient to describe how beautiful it is……must c it. Anyway, we are wondering whether it is not a surprising observation or not. I would like to have advice on our findings. Briefly, we found the following thing so far; 1) collagen network is lattice-patterned. 2) collagen-producing cells are also lattice patterned. 3) The lattice patterned collagen network cannot be restored after skin wounding. 4) Regeneration inducers can induce reformation of the network.
Axolotls, Ambystoma mexicanum, have a remarkable organ-level regeneration ability. Their regeneration ability has fascinated scientists for a long time since mammals, including humans, do not have it. The biggest issue in this field was “what are the inductive molecules?” This proposition was conceptually made in 1823. We recently succeeded in identifying the inductive molecules by using a unique experimental model. The identified molecules are BMP2+FGF2+FGF8. These regeneration inducers actually can induce organ-level regeneration in not only limbs but also the tail, teeth, and gills. Moreover, the molecules can induce limb regeneration in newts, Pleurodeles waltl, and froglets, Xenopus laevis. Indeed, our defined molecules are the regeneration inductive molecules for multiple species and multiple organs. Due to the achievement, a comparative approach can be taken at the molecular level among axolotl organs. Base on the achievement as above, we have just started investigating axolotl skin regeneration. Skin regeneration has been a gigantic research field. But unexpectedly, collagen synthetic cells in the dermis appear not to be well clarified in any species as far as we googled and PubMed-surfed. We started our research from describing collagen synthetic cells in the axolotl skin. And……My vocabulary is not sufficient to describe how beautiful it is……must c it. Anyway, we are wondering whether it is not a surprising observation or not. I would like to have advice on our findings. Briefly, we found the following thing so far; 1) collagen network is lattice-patterned. 2) collagen-producing cells are also lattice patterned. 3) The lattice patterned collagen network cannot be restored after skin wounding. 4) Regeneration inducers can induce reformation of the network.
Bio
2006 Ph.D in Tohoku University JAPAN
2006-2009 University of California Irvine, postdoc
2009- Okayama Univ, Assistant Prof
2011- Okayama Univ. Associate Prof
2006 Ph.D in Tohoku University JAPAN
2006-2009 University of California Irvine, postdoc
2009- Okayama Univ, Assistant Prof
2011- Okayama Univ. Associate Prof
Chairs
Aiko SADA, Kumamoto University, Japan
Kim ROBINSON, SRIS, Singapore
Aiko SADA, Kumamoto University, Japan
Kim ROBINSON, SRIS, Singapore